Effect of FABP6 knockdown on cell migration and invasion in BC cells. (A) Cell migration was detected using wound healing assays in siNeg and siFABP6 groups 72 h post-transfection. 8 h after scratching, the wound area was captured and analyzed using ImageJ software. (B) Cell invasion was detected using Transwell migration assays using Matrigel. After siFABP6 transfection for 72 h, T24 cells were incubated in the upper chamber. After cells were seeded on the Matrigel for 24 h, cells in the lower chamber were observed by Coomassie blue staining and counted using ImageJ software. (C,D) The expression of integrin β1, p-FAK, FAK, p-paxillin, and paxillin was analyzed after FABP6 knockdown for 72 h. GAPDH was a loading control. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to siNeg group.