HPLC-UV |
high performance liquid chromatography with ultraviolet detection |
UV |
ultraviolet |
PLGA |
poly(D,L-lactic-co-glycolic) acid |
DX |
dexamethasone |
MEL |
melatonin |
MSs |
microspheres |
DX-MEL-MSs |
dexamethasone and melatonin co-loaded PLGA microspheres |
RGC |
retinal ganglion cells |
IOP |
intraocular pressure |
LC/MS |
liquid chromatography tandem mass spectrometry |
MeOH |
methanol |
DCM |
dichloromethane |
PVA |
polyvinyl alcohol |
blank-MSs |
unloaded microspheres |
EE |
encapsulation efficiency assay |
SD |
standard deviation |
PBS |
phosphate buffer solution |
IVR |
in vitro release assay |
SS |
stock standard solution |
SS-EE |
stock standard solution for encapsulation efficiency assay |
SS-IVR |
stock standard solution for in vitro release assay |
WS |
working solution |
WS-EE |
working solution for encapsulation efficiency assay |
WS-IVR |
working solution for in vitro release assay |
ICH |
International Conference on Harmonization |
FDA |
Food and Drug Administration |
RSD |
relative standard deviation |
LOD |
limit of detection |
LOQ |
limit of quantification |
tR |
retention time |
tW |
peak width |
Rs |
resolution between peaks |
IR |
injection repeatability |
T |
tailing factor |
N |
theoretical plate number |
pS |
peak symmetry |
SEM |
scanning electron microscopy |