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. 2022 Jan 26;14(2):288. doi: 10.3390/pharmaceutics14020288
HPLC-UV high performance liquid chromatography with ultraviolet detection
UV ultraviolet
PLGA poly(D,L-lactic-co-glycolic) acid
DX dexamethasone
MEL melatonin
MSs microspheres
DX-MEL-MSs dexamethasone and melatonin co-loaded PLGA microspheres
RGC retinal ganglion cells
IOP intraocular pressure
LC/MS liquid chromatography tandem mass spectrometry
MeOH methanol
DCM dichloromethane
PVA polyvinyl alcohol
blank-MSs unloaded microspheres
EE encapsulation efficiency assay
SD standard deviation
PBS phosphate buffer solution
IVR in vitro release assay
SS stock standard solution
SS-EE stock standard solution for encapsulation efficiency assay
SS-IVR stock standard solution for in vitro release assay
WS working solution
WS-EE working solution for encapsulation efficiency assay
WS-IVR working solution for in vitro release assay
ICH International Conference on Harmonization
FDA Food and Drug Administration
RSD relative standard deviation
LOD limit of detection
LOQ limit of quantification
tR retention time
tW peak width
Rs resolution between peaks
IR injection repeatability
T tailing factor
N theoretical plate number
pS peak symmetry
SEM scanning electron microscopy