Table 1.
Proteins significantly altered in quantity between the culture supernatants of (a) tcsE mutant (M) and wildtype (WT) P. sordellii ATCC9714 strains and (b) complemented tcsE mutant (C) and the tcsE mutant vector control (VC) P. sordellii ATCC9714 strains.
| (a) | M vs. WT | C vs. VC | |||||
|---|---|---|---|---|---|---|---|
| Protein | Protein ID | Fold Change |
p-Value (×10−3) |
q-Value | Fold Change |
p-Value (×10−3) |
q-Value |
| 50S ribosomal protein L31 | CEJ72251.1 | 0.12 | 0.180 | 0.224 | 0.70 | 541 | 0.679 |
| 50S ribosomal protein L24 | CEJ72165.1 | 0.38 | 0.847 | 0.126 | 1.06 | 648 | 0.836 |
| TcsL—Lethal Toxin | CEJ75463.1 | 0.48 | 0.641 | 0.111 | 2.25 | 1.73 | 0.147 |
| 50S ribosomal protein L5 | CEJ72166.1 | 0.51 | 1.17 | 0.103 | 0.96 | 779 | 0.896 |
| probable polysaccharidede acetylase | CEJ75226.1 | 0.51 | 1.41 | 0.104 | 0.90 | 626 | 0.802 |
| 50S ribosomal protein L10 | CEJ72145.1 | 0.53 | 1.27 | 0.092 | 0.80 | 314 | 0.547 |
| 50S ribosomal protein L22 | CEJ72159.1 | 0.54 | 0.122 | 0.131 | 0.79 | 200 | 0.435 |
| 50S ribosomal protein L4 | CEJ72155.1 | 0.62 | 0.340 | 0.106 | 0.66 | 152 | 0.353 |
| phospholipase D-nuclease domain protein | CEJ72911.1 | 4.69 | 0.967 | 0.170 | 2.47 | 26.2 | 0.158 |
| (b) | C vs. VC | M vs. WT | |||||
| Protein | Protein ID | Fold Change |
p
-Value
(×10 −3 ) |
q-Value | Fold Change |
p
-Value
(×10 −3 ) |
q-Value |
| spore coat peptide assembly protein CotJC2 | CEJ72697.1 | 2.07 | 1.586 | 0.136 | 0.91 | 753 | 0.913 |
| TcsL—Lethal Toxin | CEJ75463.1 | 2.25 | 1.731 | 0.147 | 0.48 | 0.641 | 0.111 |
| branched-chain amino acid aminotransferase | CEJ74628.1 | 2.29 | 3.446 | 0.092 | 0.94 | 560 | 0.896 |
| putative delta-lactam-biosynthetic de-N-acteylase | CEJ73318.1 | 2.37 | 3.296 | 0.123 | 2.44 | 5.69 | 0.138 |
| putative ferredoxin/flavodoxinoxido reductase, alpha subunit | CEJ75209.1 | 2.48 | 1.890 | 0.169 | 0.95 | 702 | 0.914 |
| putative pyridine nucleotide-disulphide oxidoreductase | CEJ73686.1 | 2.62 | 4.003 | 0.111 | 1.09 | 653 | 0.899 |
| dimethylamine corrinoid protein | CEJ74389.1 | 2.77 | 0.808 | 0.131 | 2.50 | 132 | 0.643 |
| putative ferredoxin/flavodoxinoxido reductase, beta subunit | CEJ75208.1 | 2.86 | 4.135 | 0.139 | 1.15 | 402 | 0.896 |
| hypothetical protein | CEJ73866.1 | 3.77 | 0.002 | 0.068 | 1.23 | 639 | 0.881 |
| Chorismite mutase | CEJ73786.1 | 4.10 | 7.106 | 0.085 | 0.82 | 776 | 0.914 |
| conserved hypothetical protein | CEJ72332.1 | 5.12 | 0.711 | 0.138 | 3.47 | 14.7 | 0.185 |
| hypothetical protein | CEJ74906.1 | 6.91 | 8.456 | 0.101 | 1.40 | 766 | 0.911 |
| ureE urease accessory, N-terminal domain protein | CEJ73734.1 | 6.93 | 1.349 | 0.132 | 0.88 | 571 | 0.892 |
The proteins and their protein ID accession numbers are listed in the order of fold change. Given are the average fold change, p-value, and q-value determined by a two-tailed student t-test of log2 transformed intensity values, obtained by mass spectrometry-based label-free protein quantification. The value for the comparison of the (a) complemented tcsE mutant (C) to the tcsE mutant containing the vector control (VC), or (b) tcsE mutant (M) to the wildtype (WT), for these proteins is also provided. The only protein identified to be altered in both comparisons, and its corresponding values, is shown in bold.