FIG. 2.

(A) Western blotting performed on liver extracts from wild-type and TGase-knockout mice with anti-TGase 2 antibody. Thirty micrograms of total protein was loaded in each lane. Recombinant guinea pig TGase 2 (0.1 μg) was used as a positive control (C). This blot is representative of experiments performed on different tissues from four different animals per group. (B) TGase enzymatic activity measured in extracts from different mouse tissues and cultured MEFs in both wild-type and TGase-knockout mice. Activity was measured as incorporation of [³H]putrescine into casein. Standard deviations for 10 different evaluations are shown. (C) RT-PCR performed on RNA extracted from thymus tissues of wild-type and TGase-knockout mice using primers for TGase 2 and TGase 1.