Table 2.
The effect of eleven mutations on the measured peak densities, number of cells used for the measurements, and the concentration of VTD required to induce a 10% late current (% of peak current). The standard errors of the mean could not be calculated for the measurements or for the measurements where the necessary concentration was larger than 50 µM.
| Mutation | Peak Density (Norm. to WT) | Number of Cells | [Veratridine] for 10% Late Current (µM) ± S.E. |
|---|---|---|---|
| WT | 100 ± 6.4 | 15 | 8.2 ± 0.4 |
| E417A | 111.5 ± 14.6 | 7 | 7.8 ± 0.6 |
| E417R | 94 ± 9.6 | 9 | 31.2 ± 1.9 |
| L409S | 61.3 ±16 | 2 | >50 |
| F942A | 48.2 ± 5.1 | 6 | 10.8 ± 0.9 |
| F942Y | 88.5 ± 8 | 7 | 11.0 ± 0.8 |
| L1462S | 4.2 ± 0.7 | 1 | 6.9 |
| F1465Y | 50 ± 5.7 | 5 | 24.5 ± 2.1 |
| I1466T | 117.7 ± 12.8 | 5 | >50 |
| F1760A | 59.4 ± 8 | 6 | 7.6 ± 0.5 |
| F1760Y | 15.2 ± 1.6 | 1 | 18.6 |
| I1771T | 54.6 ± 7.3 | 8 | 9.8 ± 0.8 |