Figure 1.

Flow cytometric analysis of the camel leukogram. (A) Gating strategy for the identification of camel leukocyte populations. Singlets were excluded from the analysis based on their side scatter height (SSC-H) and SSC-Aria (SSC-A) signals. Within the mononuclear cell population, lymphocytes (L) and monocytes (M) were identified as CD14-negative and CD14-positive cells, respectively. In a SSC-A/ FL-1 dot plot, eosinophils (E) were distinguished from neutrophils (N) based on the higher green autofluorescence of their eosinophilic granules. (B) The total leukocyte number was counted under microscope using the Neubauer counting chamber after staining with Türk solution. The relative fractions of leukocyte subsets as determined using flow cytometry were used to calculate the absolute cell numbers. For both heparin and EDTA blood samples, the absolute cell numbers of all leukocyte subsets were presented as the mean and standard error of the mean. Differences between the means were calculated using the t-test and were considered significant (*) if p < 0.05.