Figure 1.

Cytopathic impact of ZIKV infection on HSerC and viral protein expression. HSerC were infected with ZIKV an MOI of 3. (A) Cytopathic impact of ZIKV infection was observed at 3, 5 and 7 days post infection (dpi) under bright−field microscopy at 200× magnification. Scale bar is 50 μm. (B) Viral protein expression was determined by Western blot using ZIKV-NS1, NS3 and ENV monoclonal antibodies at 3, 5 and 7 dpi. (C) Quantitative expression of ZIKV viral proteins determined by densitometry analysis of Western blot images using Image J and normalized to B-actin expression. (D) ZIKV proteins expression detected after 3, 5 and 7 dpi by mass spectrometry. Log2 expressions of ZIKV proteins were compared with mock-treated cells and converted to Fold Change (FC) Abbreviations. dpi = Days post infection. Exp = Expression. RFU = Relative fluorescence units.