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. 2022 Feb 11;14(2):377. doi: 10.3390/v14020377

Figure 9.

Figure 9

Western blot validation of proteomic changes detected by mass spectrometry (MS) analysis. Sertoli cell lysates were collected after ZIKV infection (MOI:3) at 3, 5 and 7 dpi. In total, 20 µg of proteins were separated by SDS-PAGE gel electrophoresis. (A) Expression of CLIC1, SPARC, STAT1, STAT3 and PSMA2 were detected by Western blot using specific antibodies. (B) Expression values of the proteins from Western-blot were quantified by ImageJ V1.8.0 from three replicates and plotted side-by-side to MS expression values. Expression of protein by Western blot was normalized to B-actin expression. Abbreviations: dpi = Days post infection; WB = Western blot; MS = Mass spectrometry; ZIKV = Zika virus.