FIG. 4.

Northern blot analysis of AaGATAr mRNA levels in males and different tissues of female mosquitoes. Total RNA was extracted from different female tissues as well as from whole males (15 μg per lane). Northern blots were hybridized with a ³²P-labeled 2.5-kb fragment from the protein-coding region (including the DNA-binding domain) of the AaGATAr cDNA clone (upper panel). To control the integrity of the isolated RNA, the same blot was hybridized to A. aegypti actin cDNA (middle panel). Portions of the gel containing rRNA were stained with ethidium bromide to control for equivalent sample loading (lower panel).