FIG. 7.

In vitro-synthesized AaGATAr protein binds to upstream elements of the A. aegypti YPP genes. (A) AaGATAr protein binds to a sequence in the Vg gene. An EMSA was done with in vitro-produced AaGATAr and AaVgGATAb as probes. A complex due to specific interaction between DNA and protein is clearly detected (lane 2). Fiftyfold molar excesses of cold probe (lane 3) and AaLAP GATA binding site (lane 4) were included as specific competition. The same excess of a cold, unrelated oligonucleotide (AaVgE75) was included in lane 5. (B) Relative affinity of different AaVg GATA binding sites for AaGATAr protein. Binding of AaGATAr to Drosophila box A element in the absence (−) or in the presence of increasing amounts of cold competing probes (self-competition [DmBoxA.], AaVgGATAa, AaVgGATAb, and AaVgGATAc) was performed to compare the binding affinity of the different AaVg GATA elements. (C) GATA motifs in the upstream regulatory regions of vitellogenin used in the competition analysis.