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. 2022 Feb 18;10(2):91. doi: 10.3390/toxics10020091

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Morphological changes of mouse liver organoids treated with different concentrations of PFAS. Mouse liver organoids treated with PFOS and PFOA for 6, 24 and 48 h at 1 mM, 500 µM and 100 µM. Images show morphological changes of organoids in bright field and H/E staining. Arrows indicate dead or suffering organoids. Empty panels are due to the toxicity of the treatment that induced organoids death. Zoom areas highlight organoids cellular and nuclear damages. H/E magnification 20×; bright field magnification 4×.