Figure 4.

IFN-γ (4 CH) stimulates the phagocytosis capabilities of granulocytes. (A) Schematic representation of the experimental protocol. Human granulocytes were preincubated during 10 min with IFN-γ (4 CH), or vehicle (Veh.), or 10 µM N-formyl-methionyl-leucyl-phenylalanine (fMLP) as a positive phagocytosis inducer. Fluorescent beads (represented in green) were then added to the culture medium for an additional 45 min. The cells were then rinsed and the fluorescence was assessed by flow cytometry. P/S: penicillin/streptomycin; BSA: bovine serum albumin. (B) IFN-γ (4 CH) stimulates the phagocytosis’ capabilities. Each condition was performed in triplicate and each histogram represents the mean ± SD of the fluorescence as a percentage of the vehicle-treated conditions, set at 100%. The black dot line was drawn to better visualize in the graph the effect of MIM compared to its Veh. control.