Figure 7.

IFN-γ (4 CH) enhances the activation of CD3-pre-primed immune cells in vitro. (A) Representative scheme of the experimental protocol. Human PBMCs from three healthy donors were cultivated during 48 h in classical culture conditions in presence of anti-CD3 (0.5 µg/mL) plus IFN-γ (4 CH)/Veh. The cells were immuno-stained at D2 and their activation status, characterized by the CD69 expression, was analyzed by flow cytometry at D3. Each cell subpopulation (NK cells, monocytes/macrophages, granulocytes, B cells, T cells, [CD4⁺ and CD8⁺]) was discriminated according to the expression of the markers referred in the Material and Methods section. The CD69 expression was assessed within the different subpopulations: NK cells, T cells, CD4⁺ T cells, CD8⁺ T cells and B cells (B–F). Each histogram represents the mean ± SEM of the CD69 expression obtained for each individual donor as a percentage of the IFN-γ (4 CH)-treated-condition CD69 expression normalized to the Veh-condition. Each individual point (black dot) represents the average of a triplicate per donor. In all the presented graphs, black dot lines were drawn to better visualize the effect of MIM compared to its Veh. control.