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. 2022 Jan 23;12(2):166. doi: 10.3390/life12020166

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression and localization of ABCG2/Breast cancer resistance protein (BCRP) in human fetal membranes. (A) Real-time polymerase chain reaction (PCR) analysis of ABCG2 mRNA in the fetal membrane (FM) shows gene expression of ABCG2. The expression levels of ABCG2 are similar in both FM and placenta (PLA) tissues. (B) Representative immunohistochemistry images of FM and human stained for BCRP. BCRP was localized to both the amnion epithelial layer (1) and the chorion trophoblast layer (2) of the FM. The PLA staining serves as the positive control. (C) Western blots of BCRP in human FM compared to the PLA. Representative blots from n = 6 for each tissue type are shown. Data presented as mean ± SD.