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. 2022 Feb 21;14(2):457. doi: 10.3390/pharmaceutics14020457

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Size distributions of LEVs and LEVs-TMO over freeze-thaw cycles. (a) Top: Dynamic light scattering (DLS) measurements of the size distribution intensity for LEVs exposed to 0, 1, and 3 freeze-thaw cycles. Insets: number-size distribution curves. Bottom: TEM images of LEVs exposed to different numbers of freeze-thaw cycles. (b) Top: DLS measurements of the size distribution intensity for LEVs-TMO exposed to 0, 1, and 3 freeze-thaw cycles. Insets: number size distribution curves. Bottom: TEM images of LEVs-TMO of different freezing and thawing cycles. Data are presented as mean ± standard error of mean (SEM).