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. 2022 Feb 2;11(2):200. doi: 10.3390/pathogens11020200

Figure 3.

Figure 3

Effect of high (HT) and ambient (AT) temperatures on the expression of selected RNAi-associated genes in capsicum chlorosis virus (CaCV)-infected and mock-inoculated capsicum plants at 5 days post inoculation (dpi), 10, and 18 dpi. The relative gene expression was measured by RT-qPCR in inoculated leaves at 5 dpi and systemic leaves at 10 and 18 dpi. The expression of (A) DCL2, (B) DCL4, (C) RdRp1, (D) RdRp6, (E) AGO1a, (F) AGO1b, and (G) AGO2 was analyzed with the 2−ΔCt method. Actin was used as internal control. The bars represent the means (±standard the error of the mean) of six biological replicates. The significance of the differences between treatments was assessed with Student’s t-test (* p < 0.05; ** p < 0.01; ns, not significant).