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. 2022 Feb 11;14(4):756. doi: 10.3390/nu14040756

Table 1.

Oligonucleotide sequence of primers for RT-PCR.

Gene Forward Primer Reverse Primer T° Annealing
Fas-L 5′-CAAGTCCAACTCAAGGTCCATGCC-3′ 5′-AGAGAGAGCTCAGATACGTTTGAC-3′ 58 °C
Bim 5′-AACCTTCTGATGTAAGTTCT-3′ 5′-GTGATTGCCTTCAGGATTAC-3′ 58 °C
p27 5′-TGCGAGTGTCTAACGGGAG-3′ 5′-GTTTGACGTCTTCTGAGGCC-3′ 59 °C
Bcl-2 5′-ATGTGTGTGGAGAGCGTCAA-3′ 5′-AGAGACAGCCAGGAGAAATCA-3′ 47 °C
BDNF 5′-GGCTGACACTTTTGAGCACGTC-3′ 5′-CTCCAAAGGCACTTGACTGCTG-3′ 52 °C
IL-1β 5′-TCATGGGATGATGATAACCTGCT-3′ 5′-CCCATACTTTAGGAAGACACGATT-3′ 50 °C
IL-6 5′-CTGGTGACAACCACGGCCTTCCCT-3′ 5′-ATGCTTAGGCATAACGCACTAGGT-3′ 54 °C
TNF-α 5′-AGCCCACGTCGTAGCAAACCA-3′ 5′-GCAGGGGCTCTTGACGGCAG-3′ 53 °C
Β-actin 5′-CGGGATCCCCGCCCTAGGCACCAGGGT-3′ 5′-GGAATTCGGCTGGGGTGTTGAAGGTCTCAAA-3′ 60 °C