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Immunohistochemical demonstration of mast cell nerve interaction in the epithelium of the suprabranchial chamber of Clarias gariepinus. Section stained for Pis 1 (in red) and 5HT (in green) showing the close anatomical association of Pis-positive nerves (arrowheads) with Pis-positive mast cells (arrows). Eosinophils (E) showing 5HT immunoreactivity. Scale bar = 20 µm.
