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Vaccine shedding in stool after multiple oral vaccinations with ShigETEC. Stool samples were obtained as available from subjects who received (A) two, (B) three or (C) 4 doses of 5 × 1010 CFU ShigETEC and were assessed for the presence of vaccine strain by PCR and culturing. PCR was considered invalid, when two independent 16S rDNA PCR assays yielded negative results and repeated DNA extraction was not possible due to small sample amount. Each line corresponds to one subject.
