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. 2022 Feb 17;23(4):2248. doi: 10.3390/ijms23042248

Figure 4.

Figure 4

Effect of exogenous competitive and oxidative inhibitors on transglutaminases. (A) The competitive inhibitor ERW1041 selectively reduced TG2 activity at a dose of 100 µM. The competitive mTG-blocker only showed minor inhibition of TG2 at a dose of 100 µM. (B) mTG activity was not decreased by the competitive TG2 inhibitor ERW1041. Competitive mTG-blocker showed a strong and dose-dependent inhibition of mTG-mediated. (C) TRX was used at a 50-fold molar excess. Thioredoxin-mediated activation of TG2 could be abolished by PX12 at doses 2 µM and 20 µM. PX12 displayed a direct inhibitory effect on TG2 activity at 2 µM and 20 µM. L-cystine significantly reduced TG2 activity at all doses tested. (D) PX12 and L-cystine did not show any significant dose-dependent effect on microbial transglutaminase (mTG) activity. Data are shown as mean ± standard deviation.* p < 0.05, ** p < 0.01, *** p < 0.001.