Figure 2.

CD44^high and CD133^high cell-derived organoids have more proliferating cells as compared to CD44^low and CD133^low organoids. (A) Sorting strategy and the level of CD44, CD133, and PTK7 in the sorted populations. (B) Number of organoids produced from cells sorted for low and high levels of the respective markers, counted on day7 (1–2 sorting experiments from organoid lines #1–#4). (C) The diameter of the organoids 7 days after seeding the sorted cells into Matrigel. (D) The percentage of KI67+ proliferating cells in the organoids (confocal microscopic images). For C and D, quantification (left panel) and representative images (right panel) are shown (two sorting experiments from four organoid lines). Scale bars: 25 µm (C,D). Mann–Whitney U test was used (B–D). ** p < 0.01, and *** p < 0.005.