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. 2022 Jan 21;12(2):157. doi: 10.3390/life12020157

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Generation and characterization of αB7-H3/CD3 bispecific antibody. (a) An illustrative representation of the αB7-H3/CD3 format. The format comprised an anti-CD3 scFv fused to light chain of a monovalent anti B7-H3 via a (G₄S)₃ linker; (b) Coomassie blue-stained SDS-PAGE analysis of purified αB7-H3/CD3 containing three chains with the following molecular weights: 57, 55, and 28 kDa; (c–k) αB7-H3/CD3 bispecific antibody dose-dependently binds to B7-H3+ tumor cells and CD3+ T cells, evaluated using flow cytometry. MFI values (control/maximum concentration of 10-2#c) of these cell lines were, respectively, 205/31,800, 7500/226,444, 480/5700, 350/27,950, 150/8065, 480/1550, 125/155, 50/10,300, and 195/3100.