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Evaluation of the interference of different tissues of juvenile shrimp on the amplification of WSSV pathogenic genes. A DirectGO direct PCR kit was the tested reagent. A 140 bp PCR amplification product of the VP28 gene was generated using the WSSV primer pair and 250 copies of standard DNA plasmid (pUC57-VP28). M: DNA marker; PW: ddH2O; T1: pereiopods; T2: pleopods; T3: gill; T4: hemolymph; T5: hepatopancreas; T6: stomach; and T7: midgut.
