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Detection of WSD, AHPND, and HPM pathogens using direct a PCR-based capillary electrophoresis platform. (A) WSD, (B) HPM, and (C) AHPND. HP: analysis of direct PCR products in the presence of hepatopancreatic lysates; PW: control group, absence of hepatopancreatic lysate; NC: negative control, absence of hepatopancreatic lysate and DNA polymerase. The PCR template was 250 copies of standard DNA plasmid.
