Table 3.
Procedure | IQ2000 | DirectGO |
---|---|---|
Pre-PCR | ||
Sample treatment | ~50 min | ~20 min |
PCR Premix | ~5 min | ~5 min |
PCR | ||
1st PCR | 21 min | 50 min 20 s |
PCR mature prepare | ~5 min | - |
2nd PCR | 30 min 10 s | - |
Post-PCR | ||
Make agarose gel | 30 min | - |
Electrophoresis | 35 min | ~3 min |
Gel stain and destain | 20 min | - |
Data assay | 5 min | 1 min |
Total time | >201 min | >86 min |