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. 2022 Feb 13;12(2):276. doi: 10.3390/life12020276

Table 3.

Comparison of the time cost for WSSV disease detection by the IQ2000TM WSSV detection system and direct PCR-based capillary electrophoresis.

Procedure IQ2000 DirectGO
Pre-PCR
Sample treatment ~50 min ~20 min
PCR Premix ~5 min ~5 min
PCR
1st PCR 21 min 50 min 20 s
PCR mature prepare ~5 min -
2nd PCR 30 min 10 s -
Post-PCR
Make agarose gel 30 min -
Electrophoresis 35 min ~3 min
Gel stain and destain 20 min -
Data assay 5 min 1 min
Total time >201 min >86 min