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. 2022 Feb 13;23(4):2063. doi: 10.3390/ijms23042063

Figure 1.

Figure 1

Heat stress reduces root meristem size in Arabidopsis thaliana. (A) Five-day-old wild-type (WT) seedlings grown on 1/2 MS medium were transferred to 22 °C and 30 °C and cultivated vertically for 3 d and 4 d and photographed. Dashed lines show the initial positions of root tips after being transferred. (B) Quantification of the length of newly grown primary roots in A, n >  34. (C) Quantification of the growth rate in A. The data are means ± SE. (D) Five-day-old roots of WT were transferred to 22 °C and 30 °C for 48 h and stained with propidium iodide to visualize the cell walls. Double arrows indicate the meristem length (from the cortical initial cell to the first elongated cell in the cortex layer). Scale bars = 100 μm. (E) Quantification of the root meristem length in D, n > 20. (F) Quantification of the cell number of meristem in D, n > 20. In the box plots (B,E,F), the boxes indicate the first and third quartiles, and the whiskers indicate the minimum and maximum values. The black lines within the boxes indicate the median values. Outliers are shown as dots. ** p < 0.01; * p < 0.05 (two-tailed, two-sample t-test).