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. 2022 Feb 16;23(4):2163. doi: 10.3390/ijms23042163

Figure 6.

Figure 6

Cells (green) were cultured on GFR Matrigel under normal gravitational (AC) and microgravity conditions (DF) for 24 h. A, D represent the confocal maximum projection. B, E represent 3D projection. C, F show the depth analysis. As shown in this figure, cells maintained at unit gravity invaded the matrix to a depth of about 390.06 µm (C), while cells maintained under a microgravity condition were able to invade to a depth of about 150 µm (F). The graph indicates the mean value ± SE of depth along the zeta axis measured at a 4-spatial series. (** 1 g versus RPM p < 0.01; scale bar 150 µm).