Expression levels of ten house-keeping genes in Phthorimaea operculella. The mean Ct values for 10 candidate reference genes are shown in three independent experiments: developmental stage, tissue, and temperature. Each box indicates the 25th and 75th percentiles. The line across the box represents the median. Abbreviation: ACT, actin; α-TUB, α-tubulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EF1α, elongation factor 1α; 18S and 28S, 18S and 28S ribosomal RNA; RPL4, RPL13 and RPL27, ribosomal protein; SOD, superoxide dismutase. The abbreviations are exactly the same as Figure 2, Figure 3, Figure 4 and Figure 5.