Table 2.

Primers of 10 candidate house-keeping genes used in qRT-PCR.

Gene	Primer Sequences (5′ to 3′)	Length (bp)	Slope	R2	Efficiency (%)
ACT	F-AATTGTGCGAGACGTCAAGG	239	−3.480	0.998	93.80
	R-CGTCGCACTTCATGATGGAG
α-TUB	F-CACTGGTAAAGAAGACGCGG	194	−3.241	0.999	103.49
	R-AGAGACGTTCCATCAGCAGG
18S	F-CGTTTGCTGGGAAGTTGACC	199	−3.289	0.997	101.39
	R-GACACGACCGTAAACCCATC
28S	F-GATTCAGTTTCGGGCACTCG	154	−3.232	0.999	103.89
	R-CTAGACCGACGCTCCATCC
GAPDH	F-TGCCACCCAAAAGACTGTTG	240	−3.338	0.998	99.33
	R-ACCTTGGCTTTGATCGCATC
EF1α	F-TGTCAAGCAGCTGATCGTTG	164	−3.286	0.999	101.52
	R-CTCCGTGCCATCCAGAAATG
RPL4	F-GGTCTGACGTGCTCAAGGTA	183	−3.452	0.991	94.84
	R-GCAGGTTCAGCTTGTCAACA
RPL13	F-AACCAACCCGCTAGAAGACA	97	−3.294	0.999	101.18
	R-CCACAGGTCTCAATGGTCCA
RPL27	F-TGAAGAACTACGACGAGGG	199	−3.384	0.992	97.47
	R-TCGAAGCTGAAGTCTACGGA
SOD	F-CAACCTGTCTCCCTGCAAAA	159	−3.328	0.998	99.75
	R-TTCGCCAACTTGTTGTAGCC