FIG. 3.
Regulation of pseudohyphal development by CDC42 alleles. Shown is the growth on nitrogen starvation medium of diploid strains expressing wild-type CDC42 (wt) or mutant CDC42 alleles leading to single-amino-acid substitutions as indicated. Plasmids carrying the different CDC42 alleles were introduced into the diploid cdc42Δ/cdc42Δ strain RH2199 by plasmid shuffling. Pseudohyphal development of resulting strains was photographed after 4 days of growth on SLAD+Ura medium. Bar, 100 μm.
