FIG. 8.

Two-hybrid protein interactions between Cdc42 proteins and effector proteins Gic1p, Gic2p, Bni1p, Ste20p, Cla4p, and Skm1p. All Cdc42p proteins measured (wild-type [wt] or mutant proteins N26I, A30T, I46M, D65N, R68S, S71P, N92D, E95K, E100G, S158T, and L160P) carry the C188S mutation to prevent plasma membrane localization. Bars and values show relative β-galactosidase activities normalized to the activities obtained for wild-type Cdc42p (wt) and the different effectors, with values set to 100 (for absolute units, see Materials and Methods). The value shown for each interaction represents the average of at least four independent β-galactosidase assays, each done in triplicate.