TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pME1758 | Cassette for full deletion of CDC42 | This study |
| pME1533 | Derivative of pRS316 with mutated CEN region | J. Hegemann |
| pME1534 | CDC42 in pTF27 | This study |
| YCp(CDC42Sc) | CDC42 in pRS315 | 65 |
| pME1552 | CDC42N26I in pRS315 | This study |
| pME1555 | CDC42A30T in pRS315 | This study |
| pME1538 | CDC42I46M in pRS315 | This study |
| pME1557 | CDC42D65N in pRS315 | This study |
| pME1548 | CDC42R68S in pRS315 | This study |
| pME1536 | CDC42S71P in pRS315 | This study |
| pME1560 | CDC42N92D in pRS315 | This study |
| pME1559 | CDC42E95K in pRS315 | This study |
| pME1551 | CDC42E100G in pRS315 | This study |
| pME1545 | CDC42S158T in pRS315 | This study |
| pME1546 | CDC42L160P in pRS315 | This study |
| pME1759 | PSTE20-GFP-STE20 fusion in pRS316 | This study |
| pME1760 | PSTE20-GFP-STE20 fusion in pRS426 | This study |
| B3782 | FLO11-lacZ reporter construct | 54 |
| pEG202 | Vector for construction of LexA fusion proteins | 21 |
| pME1913 | LexA-CDC42C188S in pEG202 | 49 |
| pME1914 | LexA-CDC42N26I,C188S in pEG202 | This study |
| pME1915 | LexA-CDC42A30T,C188S in pEG202 | This study |
| pME1916 | LexA-CDC42I46M,C188S in pEG202 | This study |
| pME1917 | LexA-CDC42D65N,C188S in pEG202 | This study |
| pME1918 | LexA-CDC42R68S,C188S in pEG202 | This study |
| pME1919 | LexA-CDC42S71P,C188S in pEG202 | This study |
| pME1920 | LexA-CDC42N92D,C188S in pEG202 | This study |
| pME1921 | LexA-CDC42E95K,C188S in pEG202 | This study |
| pME1922 | LexA-CDC42E100G,C188S in pEG202 | This study |
| pME1923 | LexA-CDC42S158T,C188S in pEG202 | This study |
| pME1924 | LexA-CDC42L160P,C188S in pEG202 | This study |
| pJG4-5 | Vector for construction of B42 transcription activation domain (B42AD) fusions | 21 |
| pJG4-5(GIC1) | B42AD-GIC1 | 6 |
| pJG4-5(GIC2) | B42AD-GIC2 | 6 |
| pJG4-5(BNI1 1-1214 aa) | B42AD-BNI1(1-1214 aa) | 13 |
| pJG4-5(STE20) | B42AD-STE20 | This study |
| pJG4-5(CLA4) | B42AD-CLA4 | 9 |
| pJG4-5(SKM1) | B42AD-SKM1 | 49 |