TABLE 3.

Regulation of growth and development by different CDC42 alleles

Developmental phenotypea	Mutation	Growth rateb (h−1)
Haploid invasive growth
+	None	0.34
−	I46M	0.34
−	S71P	0.33
Diploid pseudohyphal growth
+	None	0.37
−	N26I	0.39
++	A30T	0.32
++	D65N	0.38
+/−	R68S	0.39
++	N92D	0.36
++	E95K	0.38
−	E100G	0.36
−	S158T	0.33
−	L160P	0.38

^aHaploid invasive growth was determined by expression of CDC42 alleles in a haploid cdc42Δ mutant strain and growth on solid SC−Leu medium for 4 days before performing a wash assay. +, wild-type invasiveness; −, complete loss of invasive growth. Pseudohyphal growth was determined by expression of CDC42 alleles in a diploid cdc42Δ/cdc42Δ mutant strain and growth on solid SLAD+Ura medium for 5 days. Symbols indicate the degree of filamentation: +, wild type; ++, enhanced; +/−, reduced; −, undetectable. 

^bGrowth rate was determined in liquid SC−Leu medium.