Figure 1.

Schematic representation of the experimental approach using BMDM and PM. C57BL/6 mice were euthanized, and femur, tibia, and peritoneal cells were collected. Bone marrow cells were differentiated to macrophages (BMDM). Peritoneal macrophages (PM) were purified by adherence. BMDM and PM were primed with BCG or rBCG-S1PT for 1 day, and the supernatants were collected to determine the primary cytokine response. The cells were then left to rest for 6 days. Before the addition of the secondary stimuli (challenge), the supernatant was collected to measure glucose and lactate levels (metabolism analysis). After 1 day of re-stimulation with S. aureus, C. albicans, or LPS, the supernatants were collected to determine the secondary cytokine response.