Figure 4.

rBCG-S1PT induces metabolic changes in memory macrophages. BMDM (A) and PM (B) from naïve mice were exposed in vitro to culture medium alone (medium), BCG, or rBCG-S1PT (MOI 0.1:1), then left to rest in fresh medium for 6 days. Glucose and lactate levels and the lactate/glucose (L/G) ratio were compared between groups. Statistical analysis was performed via Mann–Whitney U test. * p < 0.05, ns = not significant. Boxes and whiskers represent value distribution with median (horizontal line), first, and third quartile (box limits) and minimum and maximum values (whiskers) of four replicate samples for BMDM and six replicate samples for PM.