Figure 4.

Control (A,B), 3H (C–E) and 24H (F–J) are representative images of the tissues collected from vehicle treated animals (n = 6) and those collected from animals at 3 h (n = 3) and 24 h (n = 3) post-ammonia treatment, respectively. Pictures B,D,E,G,H,J are the enlarged image of the tissue sections highlighted in white boxes in A,C,D,F,G,I, respectively. E,H are the highest magnification views of the white boxes from D,G, respectively. Arrowheads indicate blood vessels, while black arrows indicate the tight junctions between the epithelial cells. White arrows show the near basal membrane swelling in these epithelial cells and the arrowheads indicate epithelial cell swelling. Double arrowheads indicate separated epithelial cells. Scale bars in C,F,I = 10 μm, in A,D,G = 5 μm and in B,E,H,J = 2 μm.