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. 2022 Feb 15;14(2):395. doi: 10.3390/v14020395

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Timelines of simultaneous and sequential infections of nasal human airway epithelium (HAE) with SARS-CoV-2 and A(H1N1)pdm09 or RSV-A2 virus combinations. Nasal HAE was simultaneously or sequentially (24 h apart) infected with SARS-CoV-2/influenza A(H1N1)pdm09 or SARS-CoV-2/RSV-A2 virus combinations at the apical pole. Matched single virus infection conditions were performed in parallel. Viral loads of SARS-CoV-2, A(H1N1)pdm09 and RSV-A2 were determined by RT-qPCR in apical pole washings harvested every 24 h. Key: SARS-CoV-2, influenza A(H1N1)pdm09 virus (pH1N1) and RSV-A2 are represented in red, blue and yellow, respectively.