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Plant regeneration from 84K poplar calli. (A) Leaves of three-week-old plants used for inducing callus formation. (B) Calli induced from leaves after 6 weeks on callus induction medium 1 (CIM1). (C) Induced calli for shoot induction on shoot induction medium 1 (SIM1). (D) Shoots formed from calli after 5 weeks of growth on SIM1. (E) Shoots rooting on RM. (F) The regenerated plants obtained after 2 weeks of growth on rooting medium (RM).
