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Staining of new bone formation by aniline blue. Diminished formation of new bone in tibial defects of mice with diabetes (db−/db−) was recovered by local Marimastat therapy seven days post operation. Aniline blue positive pixels were extracted and quantified. Improvement of bone formation was observed (2.4× times). Values are depicted as ±SD. p-value: ** < 0.01. Scale bar: 200 μm.
