Figure 4.

Photomicrograph of hematoxylin and eosin-stained cerebral cortices tissue sections of: (a–d) control rats, (a) young male, (b) young female, (c) adult male, and (d) adult female, showing normal histological picture. (e) DHQ young male showing shrunken necrotic neurons with pyknotic nuclei (black arrow) and focal gliosis (red arrow). (f) DHQ young female showing necrosis of neurons (black arrow) and demyelination of nerve fibers (red arrow). (g) DHQ adult male showing congestion of cerebral blood vessels (black arrow) and necrosis of neurons with neuronophagia (red arrow). (h) DHQ adult female showing massive neuronal degeneration (black arrow) with formation of neurofibrillary tangles (red arrow) (scale bar 25 um). Graphical representation of histopathological scores for (i) degeneration and necrosis of neurons, (j) neuro fibrillary tangles, (k) focal gliosis, and (l) demyelination of nerve fibers. Each bar with a vertical line represents the mean ± S.D. of 5 rats per group. a vs. control young male, b vs. control young female, c vs. control adult male, d vs. control adult female, e vs. treated young male, f vs. treated young female, and g vs. treated adult male using three-way ANOVA followed by Tukey’s post hoc test; p < 0.05. DHQ; diiodohydroxyquinoline.