Figure 5.

Photomicrograph of hematoxylin and eosin-stained striatum tissue sections of: (a–d) control rats (a) young male, (b) young female, (c) adult male, and (d) adult female, showing the normal histological picture. (e) DHQ young male showing shrunken, atrophied and pyknotic neurons (black arrow) and demyelination (red arrow). (f) DHQ young female showing necrosis of neurons (black arrow) and severe demyelination with spongiosis of striatum (red arrow). (g) DHQ adult male showing necrosis of neurons (black arrow) and severe demyelination (red arrow). (h) DHQ adult female showing diffuse gliosis (black arrow) (scale bar 50 um). Graphical representation of histopathological scores for (i) shrunken and pyknotic neurons, (j) demyelination, (k) spongiosis, and (l) diffuse gliosis. Each bar with a vertical line represents the mean ± S.D. of 5 rats per group. a vs. control young male, b vs. control young female, c vs. control adult male, d vs. control adult female, e vs. treated young male, f vs. treated young female, and g vs. treated adult male using three-way ANOVA followed by Tukey’s post hoc test; p < 0.05. DHQ; diiodohydroxyquinoline.