Figure 6.

Histological hematoxylin and eosin-stained sections from spinal cords of (a–d) control rats, (a) young male, (b) young female, (c) adult male, and (d) adult female, showing the normal histological structure. (e) DHQ young male showing Wallerian degeneration (black arrow), demyelination (red arrow), and diffuse microgliosis in the gray matter. (f) DHQ young female showing marked Wallerian degeneration (black arrows), necrosis, and karyorrhectic nuclear changes with microgliosis (red arrow) and central chromatolysis of motor neurons (arrowhead). (g) DHQ adult male showing Wallerian degeneration (black arrow) and severe demyelination (red arrow) in the white matter. (h) DHQ adult female showing necrosis of neurons with diffuse gliosis (black arrow) and demyelination (red arrow) (scale bar 50 um). Graphical representation of histopathological scores for (i) Wallerian degeneration, (j) necrosis of neurons, (k) demyelination in the white matter, and (l) microgliosis in the gray matter. Each bar with a vertical line represents the mean ± S.D. of 5 rats per group. a vs. control young male, b vs. control young female, c vs. control adult male, d vs. control adult female, e vs. treated young male, and f vs. treated young female using three-way ANOVA followed by Tukey’s post hoc test; p < 0.05. DHQ; diiodohydroxyquinoline.