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. 2022 Feb 19;15(2):251. doi: 10.3390/ph15020251

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histological Luxol fast blue-stained sections from spinal cords of (a–d) control rats, (a) young male, (b) young female, (c) adult male, and (d) adult female, showing apparently normal dark blue myelinated axons. (e–h) showing demyelination in white and gray matter (black arrow), (e) DHQ young male, (f) DHQ young female, (g) DHQ adult male, and (h) DHQ adult female. (Scale bar 50 um). (i) Quantitative analysis of LFB staining in sections from spinal cords. Each bar with a vertical line represents the mean ± S.D. of 5 rats per group (5 fields/rat). a vs. control young male, b vs. control young female, c vs. control adult male, d vs. control adult female, e vs. DHQ young male, f vs. DHQ young female, and g vs. DHQ adult male using three-way ANOVA followed by Tukey’s post hoc test; p < 0.05. DHQ; diiodohydroxyquinoline.

Histological Luxol fast blue-stained sections from spinal cords of (a–d) control rats, (a) young male, (b) young female, (c) adult male, and (d) adult female, showing apparently normal dark blue myelinated axons. (e–h) showing demyelination in white and gray matter (black arrow), (e) DHQ young male, (f) DHQ young female, (g) DHQ adult male, and (h) DHQ adult female. (Scale bar 50 um). (i) Quantitative analysis of LFB staining in sections from spinal cords. Each bar with a vertical line represents the mean ± S.D. of 5 rats per group (5 fields/rat). a vs. control young male, b vs. control young female, c vs. control adult male, d vs. control adult female, e vs. DHQ young male, f vs. DHQ young female, and g vs. DHQ adult male using three-way ANOVA followed by Tukey’s post hoc test; p < 0.05. DHQ; diiodohydroxyquinoline.