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Abrogation of MAT1 inhibits cyclin E expression in U-2 OS cells but has no effect on CDK phosphorylation. (a) Cellular cyclin D1-CDK phosphorylation of pRb. Cellular cyclin D1-CDK complexes immunoprecipitated from transduced and nontransduced U-2 OS cells were used as enzymes for the kinase assay, and GSTpRb-C was used as a substrate. (b and c) Western blotting analysis of CDK4 (b) and CDK2 (c) phosphorylation status. (d) Western blotting analysis of cyclin D1 and cyclin E expression.
