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Overview of matrix-assisted desorption/ionization mass spectrometry (MALDI-MS) imaging. First, a MALDI matrix solution is sprayed onto mounted tissue sections on indium tin oxide (ITO) glass slides. Then, the sample is irradiated with a UV pulse laser in the MALDI mass spectrometer, and the ions produced from tissue sections are detected. Finally, images of the detected ions on tissues are integrated with the obtained MALDI mass spectra.
