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. 2022 Feb 21;10(2):484. doi: 10.3390/microorganisms10020484

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Formation of the metabolic intermediates phenol and 2-hydroxymuconic semialdehyde (2-HMS) during C. metallidurans CH34 growth on benzene. (A) CH34 cells were grown in LPTMS minimal medium using benzene (5 mM) as sole carbon and energy sources. Control assays without carbon source are also depicted. (B) The metabolic intermediates were analyzed by HPLC. Benzene degradation (square), phenol formation (triangle), and the generation of 2-hydroxymuconic semialdehyde (2-HMS; circle) after meta-cleavage of the catechol ring are indicated. Control assays without bacteria showed no degradation (data not shown). Each point is an average ± SDs of results from at least three independent assays.