Table 3.
Gene | Primer Sequence (5′→3′) | Annealing | Product | Reference |
---|---|---|---|---|
(°C) | Size (bp) | Sequence | ||
FGF8 | F_ TGTCTCCCAACAGCATGTGA | 59 | 181 | NM_033164.4 |
R_ CGTCTCCACGATGAGCTTTG | ||||
PAPSS2 | F_ CAAACTTGACCACGTCCGAG | 57 | 179 | NM_004670.4 |
R_ TCACGCCATCATCTAGCAGG | ||||
CDK12 | F_ AGTCCACTCCCCAGTAGGAA | 57 | 168 | NM_016507.4 |
R_ ACTGAGTTCAGCTCCCAGAC | ||||
LDHA | F_ GAACAGTGGAAAGAGTGCAGAT | 57 | 132 | NM_001165415.2 |
R_ AGGACAACATGCACAACCTC | ||||
PRKCSH | F_ CACAATTGGGCACAGGGAG | 59 | 129 | NM_001289102.1 |
R_ GGCCTCACTTTGTTTCCGAT | ||||
CSE1L | F_ GGCACAGTCACTTCACAAGTT | 58 | 171 | NM_001362762.2 |
R_ CGCCAATACAAACCCCATCTT | ||||
STRAD13 | F_ GCCGAGATGTTCAGTCAGGT | 57 | 159 | NM_178006.4 |
R_ CACTAGCTGATGGCGTGCTA | ||||
OGDHL | F_ GTTTCTTCAAACGTGGATCTTGC | 60 | 139 | NM_001363523.2 |
R_ ATTCCTGTCCCCCGATGAAA | ||||
TLE3 | F_ CTCGCCCTTGTCAGCTCTTA | 57 | 200 | NM_001282982.2 |
R_ GTTCAGCTCCGTCATGGTGA | ||||
GAPDH | F_ GAAATGAATGGGCAGCCGTT | 59 | 173 | NM_001256799.3 |
R_ CGCCCAATACGACCAAATCAG |