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Ex vivo susceptibility of Sporothrix spp. biofilms grown on sterile cat claws. Biofilms of S. brasiliensis ATCC MYA 4823 (A) or S. schenckii ATCC 32286 (B) were grown for 120 h, and were exposed to the MIC80 of itraconazole (64 µg/mL) or miltefosine (8 µg/mL) for 48 h. Recovered biofilms were plated and the colony-forming units normalized by the biofilm weight. Control biofilms were incubated with fresh media (RPMI 2% glucose). ns, not significant; **, p < 0.01; ****, p < 0.001.
