Table 1.

MHCI binding predictions were made on 2 November 2021 using the IEDB analysis resource Consensus tool [154], which combines predictions from ANN aka NetMHC (4.0) [155,156,157], SMM [158], and Comblib [159]. For all values, higher scores indicate higher predicted efficiency, whereas a smaller MHC IC50 predicts a better binding. The processing score combines the proteasomal cleavage and TAP transport predictions. The total score combines the proteasomal cleavage, TAP transport, and MHC binding predictions. The sequences highlighted in grey show the best candidate for the indicated MHC class I complex.

Protein	AA Position	Sequence	HLA-Allele	Proteasome Score	TAP Score	MHC Score	Processing Score	Total Score	MHC IC50 [nM]
HBcAg	18	FLPSDFFPSV	A*02:01	1.44	0.12	−0.59	1.56	0.97	3.9
HBVpol	455	GLSRYVARL	A*02:01	1.53	0.37	−2.08	1.90	−0.18	121
HDAg	26	KLEDLERDL	A*02:01	1.30	0.45	−3.68	1.75	−1.92	4734
HDAg	43	KLEDENPWL	A*02:01	1.54	0.45	−2.06	1.99	−0.07	114.3
HBVpol	173	SPYSWEQEL	B*35:01	1.54	0.38	−2.36	1.92	−0.44	229
HDAg	192	QGFPWDILF	B*35:01	1.27	1.10	−2.76	2.37	−0.38	571
HDAg	194	FPWDILFPA	B*35:01	0.66	−0.34	−1.20	0.32	−0.88	15.7