Table 4.

Mediterranean fish species used to assess distribution of RGNNV reservoirs in shellfish through phylogenetics.

Classification a	Species	RNA2 sequence b	Collection Area c	Reference d	Biological Trophic Niche	Reference
Scorpaeniformes
Triglidae	Chelidonichthys lucernus	KM001708	Gulf of Cadiz	[29]	Fish, crustacean and mollusks	[41]
Scorpaenidae	Scorpaena scrofa	MK766980	Aegean Sea	[22]	Fish, crustacean and mollusks	[42]
Batrachoidiformes
Batrachoididae	Halobatrachus didactylus	KM001712	Gulf of Cadiz	[29]	Fish and crustacean	[43,44]
Beloniformes,
Exocoetidae	Cheilopogon heterurus	MK767007	Aegean Sea	[22]	Not determined
Clupeiformes
Clupeidae	Sardina pilchardus	MK766977	Aegean Sea	[22]	Fish eggs, crustacean eggs, copepods, decapods, cirripedes, dinoflagellates and diatoms.	[45,46]
		MK766998	Ionian Sea	[22]
Gadiformes
Gadidae	Trisopterus minutus	MK766999	Aegean Sea		Fish and crustacean	[47,48]
Perciformes
Sparidae	Boops boops	KM767004	Aegean Sea	[22]	Mainly on crustaceans, also planktophagous	[49]
		MK766993	Aegean Sea	[22]
	Sparus aurata	MK766992	Aegean Sea (Farm)	[22]	Shellfish, including mussels and oysters	[50]
		MK766983	Ionian Sea	[22]
		MK766997	Aegean Sea	[22]
	Pagellus erithrinus	MK767013	Ionian Sea	[22]	Mainly benthic invertebrates and small fishes, also omnivorous	[50]
	Spondyliosoma cantharus	KM001718	Gulf of Cadiz	[29]	Seaweeds and small invertebrates (crustaceans)	[50]
	Pagellus acarne	KM001716	Gulf of Cadiz	[29]	Worms, mollusks and small crustaceans, also omnivorous	[50,51]
	Pagrus pagrus	MK766996	Ionian Sea	[22]	Crustacean, fishes and mollusks	[50,52]
	Spicara smaris	MK766987	Aegean Sea	[22]	Zooplanktivorous, mainly fish larvae	[53]
	Sarpa salpa	MK766974	Aegean Sea	[22]	Plankton feeders and herbivorous	[54]
		MK766978	Aegean Sea	[22]
Moronidae	Dicentrarchus labrax	MK766976	Aegean Sea (Farm)	[22]	Fish, crustaceans and mollusks	[55]
		MK766975	Aegean Sea (Farm)	[22]
		MK766990	Ionian Sea (Farm)	[22]
		MK766985	Aegean Sea	[22]
		MK766981	Ionian Sea	[22]
		MK766988	Ionian Sea	[22]
Scianidae	Umbrina cirrosa	MK766979	Aegean Sea	[22]	Fish and benthic invertebrates, also omnivorous	[56]
Mullidae	Mullus barbatus	KM001715	Gulf of Cadiz	[29]	Small benthic crustaceans, worms and mollusks	[57]
		MK767003	Aegean Sea	[22]
		MK767005	Ionian Sea	[22]
		MK766982	Ionian Sea	[22]
	Mullus surmuletus	MK766984	Aegean Sea	[22]	Fish, crustacean and mollusks	[58]
		MK766991	Aegean Sea	[22]
Scombriformes
Sphyraenidae	Sphyraena sphyraena	MK767001	Aegean Sea	[22]	Fish, cephalopods and crustaceans	[59]
Scombridae	Scomber scombrus	MK766995	Aegean Sea	[22]	Zooplankton and small fishes	[60]

^a Phylum Order, Family. ^b GenBank accession number. ^c Area of collection of specimens from which RGNNV RNA2 sequence were obtained. ^d Reference detecting RNA2 of RGNNV in shellfish and indicated fish species.